Specialist course on digital PCR
6-10 September 2018


The third edition of the digital PCR specialist course at Ghent University was held on 6-10 September 2018. In 2019 we are organizing the fourth edition. For more information, visit the website

Course program


Date and location

The course will take place on 6, 7 and 10 september 2018 at the faculty of Veterinary Sciences, Ghent University.

Day 1

08:30-10:00 Introduction to digital PCR
Ward De Spiegelaere
10:00-10:30 Coffee break
10:30-12:00 Applications of digital PCR
Ward De Spiegelaere, Nicole Hersmus & Ide Smets
12:00-13:00 Lunch
13:00-14:30 Data driven droplet calling methods
Olivier Thas & Antoon Lievens
14:30-15:00 Coffee break
15:00-17:00 Exercise on droplet classification methods
Wim Trypsteen

Day 2

08:30-10:00 Assay design including hands on training
Jo Vandesompele
10:00-10:30 Coffee break
10:30-12:00 dPCR optimization, power analysis and quality control
Antoon Lievens & Olivier Thas
12:00-13:00 Lunch
13:00-14:30 Applications: CNV detection, genotyping and mutation detection
Jo Vandesompele & Elien Augustus
14:30-15:00 Coffee break
15:00-17:00 Mixed models for dPCR incl. hands on training
Olivier Thas

Day 3

08:30-10:00 Hands on exercise on a digital PCR platform
Bio-Rad: QX200 ddPCR System
10:00-10:30 Coffee break
10:30-12:00 RNA quantification by dPCR and normalization tools
Ward De Spiegelaere
12:00-13:00 Lunch
13:00-15:10 Hands on exercise on a digital PCR platform
Stilla Technologies: The Naica System
15:15-15:30 Coffee break
15:30-16:30 Minimal reporting guidelines
Ward De Spiegelaere

Registration


Academic researchers (PhD students, Postdocs and PIs): 45€
Non-academic researchers: 200€

Please register by completing the form available here.

How to get there


Faculty of Veterinary Medicine
Salisburylaan 133
building DI5, entrance 78, first floor
find on google maps

Contact us?


ward.despiegelaere@ugent.be