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Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA

In the present paper, we provide a step-by-step experimental protocol for triplex digital PCR assays to assess the intactness of integrated HIV DNA. These assays were validate their performance on a latently infected Jurkat cell-line model and HIV-1 patient samples.

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Featured Publications

The Digital MIQE Guidelines Update

Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Partition classification: ddpcRquant

Threshold determination for single channel droplet digital PCR experiments.

Flexible data-analysis using GLMM

Flexible analysis of digital PCR experiments using generalised linear mixed models.

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