Specialist course on digital PCR
6-10 September 2018

The third edition of the digital PCR specialist course at Ghent University was held on 6-10 September 2018. In 2019 we are organizing the fourth edition. For more information, visit the website

Course program

Date and location

The course will take place on 6, 7 and 10 september 2018 at the faculty of Veterinary Sciences, Ghent University.

Day 1

08:30-10:00 Introduction to digital PCR
Ward De Spiegelaere
10:00-10:30 Coffee break
10:30-12:00 Applications of digital PCR
Ward De Spiegelaere, Nicole Hersmus & Ide Smets
12:00-13:00 Lunch
13:00-14:30 Data driven droplet calling methods
Olivier Thas & Antoon Lievens
14:30-15:00 Coffee break
15:00-17:00 Exercise on droplet classification methods
Wim Trypsteen

Day 2

08:30-10:00 Assay design including hands on training
Jo Vandesompele
10:00-10:30 Coffee break
10:30-12:00 dPCR optimization, power analysis and quality control
Antoon Lievens & Olivier Thas
12:00-13:00 Lunch
13:00-14:30 Applications: CNV detection, genotyping and mutation detection
Jo Vandesompele & Elien Augustus
14:30-15:00 Coffee break
15:00-17:00 Mixed models for dPCR incl. hands on training
Olivier Thas

Day 3

08:30-10:00 Hands on exercise on a digital PCR platform
Bio-Rad: QX200 ddPCR System
10:00-10:30 Coffee break
10:30-12:00 RNA quantification by dPCR and normalization tools
Ward De Spiegelaere
12:00-13:00 Lunch
13:00-15:10 Hands on exercise on a digital PCR platform
Stilla Technologies: The Naica System
15:15-15:30 Coffee break
15:30-16:30 Minimal reporting guidelines
Ward De Spiegelaere


Academic researchers (PhD students, Postdocs and PIs): 45€
Non-academic researchers: 200€

Please register by completing the form available here.

How to get there

Faculty of Veterinary Medicine
Salisburylaan 133
building DI5, entrance 78, first floor
find on google maps

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