The fourth edition of the digital PCR specialist course at Ghent University will teach you the basics of digital PCR, including assay design, digital PCR optimization and data analysis. We will start with a basic intruduction of digital PCR and explain possible applications using case studies in the field of cancer research and virology. In addition to this, the course will provide a framework for good experimental practice.
The course will be taught by experts in the field of digital PCR from Ghent University, The University of Antwerp and the Catholic University of Leuven. Hands on training on dPCR devices will be provided by Bio-Rad (QX200) and Stilla Technologies (Naica system)
The course is open for academic and non academic researchers, see registration for pricing
The course will take place on 10, 11 and 12 september 2018 at the faculty of Veterinary Sciences, Ghent University.
| 08:30-10:00 | Introduction to digital PCR Ward De Spiegelaere |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | Hands on exercises on a digital PCR platform Bio-Rad |
| 12:20-12:50 | Lunch |
| 12:50-14:20 | Hands on exercise on a digital PCR platform Stilla Technologies |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Applications of dPCR Ward De Spiegelaere |
| 08:30-10:00 | Assay design including hands on training Jo Vandesompele |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | dPCR optimization, power analysis and quality control Antoon Lievens & Olivier Thas |
| 12:020-12:50 | Lunch |
| 12:50-14:20 | Partition categorization methods Olivier Thas & Antoon Lievens |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Applications: CNV detection, genotyping and mutation detection Jo Vandesompele & Patrick Pauwels |
| 08:30-10:00 | Exercises on partition categorization methods Wim Trypsteen |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | GLMM methods for dPCR data analysis Olivier Thas |
| 12:20-12:50 | Lunch |
| 12:50-14:20 | dPCR applications Nicole Hersmus RNA quantification by dPCR and normalization tools Ward De Spiegelaere |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Minimal reporting guidelines for dPCR experiments Ward De Spiegelaere |
UGent doctoral school participants: free
Non-UGent Academic researchers (PhD students, Postdocs and PIs): 45€ (fully booked)
Non-academic researchers: 250€ (fully booked)
Faculty of Veterinary Medicine
Salisburylaan 133
building DI5, entrance 78, first floor
find on google maps
ward.despiegelaere@ugent.be