Specialist course on digital PCR
9,10 & 13 September 2021

The fifth edition of the digital PCR specialist course at Ghent University will teach you the basics of digital PCR, including assay design, digital PCR optimization and data analysis. We will start with a basic intruduction of digital PCR and explain possible applications using case studies in the field of cancer research and virology. In addition to this, the course will provide a framework for good experimental practice.

The course will be taught by experts in the field of digital PCR from Ghent University anf The University of Antwerp. Hands on training on dPCR devices will be provided by Stilla Technologies (Naica system)

The course is open for academic and non academic researchers, see registration for pricing

Course program

Date and location

The course will take place on 9, 10 and 13 September 2021 at the faculty of Veterinary Sciences, Ghent University.

Day 1: Introduction & Hands on training

08:30-10:00 Introduction to digital PCR
Ward De Spiegelaere
10:00-10:20 Coffee break
10:20-12:20 Hands on exercises on a digital PCR platform
Stilla Technologies
12:20-12:50 Lunch
12:50-14:20 Assay design including hands on training
Jo Vandesompele
14:20-14:40 Coffee break
14:40-17:00 Applications of dPCR: Absolute quantification, mutation detection, CNV analysis
Jo Vandesompele, Ward De Spiegelaere& Wim Trypsteen

Day 2 assay design & applications

08:30-10:00 Interpreting dPCR data
Wim Trypsteen & Ward De Spiegelaere
10:00-10:20 Coffee break
10:20-12:20 Hands on exercise on a digital PCR platform
Stilla Technologies
12:020-12:50 Lunch
12:50-14:20 dPCR optimization, power analysis and quality control
Antoon Lievens & Olivier Thas
14:20-14:40 Coffee break
14:40-17:00 Partition categorization methods
Olivier Thas & Antoon Lievens

Day 3: data analysis & reporting

08:30-10:00 Exercises on partition categorization methods
Wim Trypsteen
10:00-10:20 Coffee break
10:20-12:20 GLMM methods for dPCR data analysis
Olivier Thas
12:20-12:50 Lunch
12:50-14:20 Applications: mutation detection in oncology
Patrick Pauwels
RNA quantification by dPCR and normalization tools
Ward De Spiegelaere
14:20-14:40 Coffee break
14:40-17:00 Minimal reporting guidelines for dPCR experiments
Ward De Spiegelaere


The course is fully booked, but UGent doctoral school participants can register on the waiting list in case a place will become free:
UGent doctoral school participants: waiting list

How to get there

Faculty of Veterinary Medicine
Salisburylaan 133
building DI5, entrance 78, first floor
find on google maps

Contact us?