The fifth edition of the digital PCR specialist course at Ghent University will teach you the basics of digital PCR, including assay design, digital PCR optimization and data analysis. We will start with a basic intruduction of digital PCR and explain possible applications using case studies in the field of cancer research and virology. In addition to this, the course will provide a framework for good experimental practice.
The course will be taught by experts in the field of digital PCR from Ghent University anf The University of Antwerp. Hands on training on dPCR devices will be provided by Stilla Technologies (Naica system)
The course is open for academic and non academic researchers, see registration for pricing
The course will take place on 9, 10 and 13 September 2021 at the faculty of Veterinary Sciences, Ghent University.
| 08:30-10:00 | Introduction to digital PCR Ward De Spiegelaere |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | Hands on exercises on a digital PCR platform Stilla Technologies |
| 12:20-12:50 | Lunch |
| 12:50-14:20 | Assay design including hands on training Jo Vandesompele |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Applications of dPCR: Absolute quantification, mutation detection, CNV analysis Jo Vandesompele, Ward De Spiegelaere& Wim Trypsteen |
| 08:30-10:00 | Interpreting dPCR data Wim Trypsteen & Ward De Spiegelaere |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | Hands on exercise on a digital PCR platform Stilla Technologies |
| 12:020-12:50 | Lunch |
| 12:50-14:20 | dPCR optimization, power analysis and quality control Antoon Lievens & Olivier Thas |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Partition categorization methods Olivier Thas & Antoon Lievens |
| 08:30-10:00 | Exercises on partition categorization methods Wim Trypsteen |
| 10:00-10:20 | Coffee break |
| 10:20-12:20 | GLMM methods for dPCR data analysis Olivier Thas |
| 12:20-12:50 | Lunch |
| 12:50-14:20 | Applications: mutation detection in oncology Patrick Pauwels RNA quantification by dPCR and normalization tools Ward De Spiegelaere |
| 14:20-14:40 | Coffee break |
| 14:40-17:00 | Minimal reporting guidelines for dPCR experiments Ward De Spiegelaere |
Faculty of Veterinary Medicine
Salisburylaan 133
building DI5, entrance 78, first floor
find on google maps
ward.despiegelaere@ugent.be